beta actin Monoclonal Antibody
Catalog No. B-IO-10026 Reactivity H,M,R,Mk,Ch,Dg,Hm,Rb
Storage Store at -20℃. Avoid freeze / thaw cycles. Host Mouse
Applications WB,IHC-p Isotype IgG
Note: Centrifuge before opening to ensure complete recovery of vial contents.
ImagesImmunogen Information
Immunogen Synthetic Peptide
Swissprot P60709
Synonyms ACTB,Actin,cytoplasmic 1,Beta-actin
Product Information
Calculated MW 42kDa
Observed MW 43kDa
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.4
Purify Protein A purification
Clone No. Clone:2M3
Dilution WB 1:2000-1:5000, IHC 1:50-1:200
Background
Beta actin, also named as ACTB and F-Actin, belongs to the actin family.
Actins are highly conserved globular proteins that are involved in various
types of cell motility and are ubiquitously expressed in all eukaryotic
cells. At least six isoforms of actins are known in mammals and other
vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal
muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1
(ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are
two non-muscle actin proteins. Most actins consist of 376aa, while
ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle
cells) has only 374aa. Beta actin has been widely used as the internal
control in RT-PCR and Western Blotting as a 42-kDa protein. However,
the 41 kDa cleaved fragment of beta actin can be generated during
apoptosis process. This antibody can recognize all the actins.
For Research Use Only
