beta actin Polyclonal Antibody
Catalog No. B-IO-10027 Reactivity H,M,R
Storage Store at -20℃. Avoid freeze / thaw cycles. Host Rabbit
Applications WB,IHC-p,IF,ELISA Isotype IgG
Note: Centrifuge before opening to ensure complete recovery of vial contents.
ImagesImmunogen Information
Immunogen Synthesized peptide derived from the N-terminal
region of human Actin β.
Swissprot P60709
Synonyms ACTB,Actin,cytoplasmic 1,Beta-actin
Product Information
Calculated MW 42kDa
Observed MW 42kDa
Buffer PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4.
Purify Affinity purification
Dilution WB 1:1000-1:4000, IHC 1:100-1:300, IF 1:100-1:300, ELISA 1:20000
Background
Beta actin, also named as ACTB and F-Actin, belongs to the actin family.Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
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